An Improved Protocol for Intact Chloroplasts and cpDNA Isolation in Conifers
نویسندگان
چکیده
منابع مشابه
An Improved Protocol for Intact Chloroplasts and cpDNA Isolation in Conifers
BACKGROUND Performing chloroplast DNA (cpDNA) isolation is considered a major challenge among different plant groups, especially conifers. Isolating chloroplasts in conifers by such conventional methods as sucrose gradient and high salt has not been successful. So far, plastid genome sequencing protocols for conifer species have been based mainly on long-range PCR, which is known to be time-con...
متن کاملIsolation of Intact Chloroplasts from Dunaliella tertiolecta.
Cells of Dunaliella tertiolecta from the log phase of growth were broken by rapid extrusion at low pressure through a Yeda press and the chloroplasts were isolated by centrifugation through a Percoll gradient. Osmolarity of the growth media, the suspending media, and the Percoll gradient was kept identical to minimize change in chloroplast volume and mitochondrial entrapment. The isolated intac...
متن کاملdevelopment and implementation of an optimized control strategy for induction machine in an electric vehicle
in the area of automotive engineering there is a tendency to more electrification of power train. in this work control of an induction machine for the application of electric vehicle is investigated. through the changing operating point of the machine, adapting the rotor magnetization current seems to be useful to increase the machines efficiency. in the literature there are many approaches wh...
15 صفحه اولAn Improved Junction-Based Directional Routing Protocol (IJDRP) for VANETs
Vehicular Ad-Hoc Networks (VANETs) is a novel technology that has recently emerged and due to its swift changing topology and high mobility nature, it has become problematic to design an efficient routing protocol in VANETs’ amongst both moving and stationary units. Also, the existing routing algorithms are not very effective to satisfy all requirements of VANETs. This paper explores the need o...
متن کاملImproved Protocol for Isolation of Genomic DNA from Leaf Tissues of Phyllanthus emblica Gaertn
Modified Cetyltrimethylammonium bromide (CTAB) protocol for DNA isolation was developed from leaf tissuesof Phyllanthus emblica for obtaining high quality genomic DNA. Fresh leaves of three different maturitywere analyzed for yield and quality of DNA. Acidity was determined in three different maturity of leaves viz.tender, intermediate and mature and their influence on DNA qua...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: PLoS ONE
سال: 2014
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0084792